The BioProfile FLEX2 On-Line Autosampler (OLS)with SRC is a modular system that allows automated sampling, analysis and cooled storage of aliquotes. The OLS consists of the FLEX2 analyzer, Sample Transfer Module (STM), Reactor Sampling Module (RSM) and the Sample Retain Collector (SRC). Fully configured, the OLS’ small sample volume and fast analysis time provide automated sampling and analysis of 10 bioreactors in less than 1.5 hours. Setup time for a full, 10-bioreactor system requires less than 20 minutes.
With proven data comparability to manual sampling methods, the FLEX2 OLS can be connected to OPC-compliant control systems for automated sampling, analysis, and feedback control. The system is compliant with GMP and 21 CFR 11. Lab-Level Validation (IQ/OQ) protocols and support are available to monitor proper system performance and functionality. Cell lines can be optimized by adding a CDV module, enabling comparable CDV results to other established cell counting systems (see CDV comparability studies). FLEX2 External Sampling Module offers direct integration with bioreactors and microbioreactors (incl. Sartorius Ambr® 15 / 250 ).
Features:
BioProfile FLEX2 analyzer
- Offers a comprehensive test panel of 16 cell culture parameters including pH, gases, metabolites, osmometry, cell density and cell viability (CDV).
- Comprises maintenance- free chemistry and gas sensors
- Enables automated liquid quality control (QC) and supplemental quality monitoring (SQM)
- Flexible sample load options through 24-external load-and-go tray, batch sampling via 96-well plate and direct sample analysis from syringes and sampling cups allowing to interupt other options for critical samples
- The STM is the main interface to the RSM.
- The STM enables sampling from up to 10 bioreactors.
- Sampling sequence time (start of sample aspiration to full sample analysis by FLEX2) is as fast as 6 minutes.
- Up to 24 samples per day (for each bioreactor) can be analyzed for a detailed look at culture performance.
- Sampling and cleaning happen simultaneously via two independent fluid paths.
- The RSM incorporates precision fluid pumps that transfer 5.6 mL of sample from the bioreactor for FLEX2 analysis.
- An RSM is installed next to each bioreactor, minimizing sample volume requirements.
- RSMs can be installed as far as 20 feet from the STM
- The RSM’s intuitive touchscreen enables each bioreactor to be individually configured for autosampling. Sampling can also be manually initiated at the RSM touchscreen.
Sample Retain Collector (SRC)- Fully automated collection and storage of sample retains from 0.5 to 50 mL.
- Uses 2mL, 15mL, or 50mL sample racks
- Fulfills regulatory requirements for long-term sample retains
- Enables further off-line testing
